منابع مشابه
Energy transfer in modified DNA.
Energy transfer at room temperature and in aqueous solution between two hydrocarbon chromophores, 2-acetylaminofluorene and 7-methylbenz(a)anthracene, covalently attached to DNA and to homopolynucleotide models is described. Transfer, as evidenced by changes in fluorescence intensity and polarization, is a function of secondary structure.
متن کاملDNA-mediated electron transfer in naphthalene-modified oligonucleotides.
Naphthalene-modified oligonucleotides have been synthesized and characterized with respect to electron transfer chemistry. Using the Sonogashira coupling reaction, naphthalene can be covalently anchored onto a modified uridine through an ethynyl linkage. This tethering allows for effective electronic coupling with the DNA bases, resulting in a significant red shift of the absorption bands of th...
متن کاملNew energy transfer dyes for DNA sequencing.
We have synthesized a set of four energy transfer dyes and demonstrated their use in automated DNA sequencing. The donor dyes are the 5- or 6-carboxy isomers of 4'-aminomethylfluorescein and the acceptor dyes are a novel set of four 4,7-dichloro-substituted rhodamine dyes which have narrower emission spectra than the standard, unsubstituted rhodamines. A rigid amino acid linker, 4-aminomethylbe...
متن کاملTriple fluorescence energy transfer in covalently trichromophore-labeled DNA.
DNA is a unique molecule that can be used to separate donor and acceptor for fluorescence energy transfer (ET)1 and longrange photoinduced electron-transfer studies.2 ET has been used extensively as a spectroscopic ruler for biological structures,3a-c and ET primers and terminators are markedly superior to single dye-labeled reagents in DNA sequencing and analysis.4a-b Molecular beacons using b...
متن کاملSequence dependence of energy transfer in DNA oligonucleotides.
The sequence, temperature, concentration, and solvent dependence of singlet energy transfer from normal DNA bases to the 2-aminopurine base in synthesized DNA oligomers were investigated by optical spectroscopy. Transfer was shown directly by a variable fluorescence excitation band at 260-280 nm. Adenine (A) is the most efficient energy donor by an order of magnitude. Stacks of A adjacent to 2A...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1971
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.68.1.205